资源植物藏茵陈的研究进展

本文对藏茵陈资源以及藏茵陈在分子生物学、指纹图谱、胚胎学、组织培养等方面的研究进展进行了综述。     

生物技术在牧草及草坪草中的研究进展及应用前景

现代生物技术在牧草及草坪植物育种工作中的运用为其打开了一个崭新的局面。组织培养和再生体系的建立为基因片段的转移和转化提供了前提条件。本文对目前遗传转化中几种较为常见的方法作了较为详细的阐述,并对其发展趋势提出了一些看法。     

硝酸钙和IAA对温州蜜柑果实钙含量及其品质的影响

在花期、花后1周、3周、采前2周对龟井蜜柑进行Ca(NO3)2、IAA和IAA+Ca(NO3)2喷布处理,同时测定了不同发育时期各处理果实钙含量及其采后果实的主要品质,并与采后浸钙处理和完熟采收果实品质进行了对比分析,结果表明:1)各处理的果皮钙含量在花后51d和112d均有所提高,但此期整个果实钙含量并无显著性差异,采收时各处理整个果实和果肉钙含量均显著高于对照;2)采收时各处理果实的可溶性固形物和可滴定酸均显著低于对照,可溶性糖、单果重、果皮色差值和相对电导率与对照无显著性差异,各处理糖酸比显著高于对照;3)采后30d时IAA+Ca(NO3)2喷布和采后浸钙处理果实的维生素C及完熟采收果实的可溶性固形物和可溶性糖均显著高于对照和其他处理。     

不同砧木嫁接网纹甜瓜试验

为预防网纹甜瓜枯萎病等土传病害,以新土佐、灰甘瓜、全能铁甲南瓜,甬砧3号甜瓜为砧木,以当地主栽的拿玻里网纹甜瓜为接穗,进行嫁接试验。结果表明:拿玻里网纹甜瓜嫁接后,植株的抗病性有了显著提高,对枯萎病的防效高达100%,对白粉病的防效也有所提高,综合抗性增强。其中甬砧3号在4个参试砧木品种中,与接穗拿玻里网纹甜瓜的亲和性最好,抗病性提高明显,品质也较自根苗好。     

宁夏春小麦磷素利用效率的基因型差异研究

在不同施磷土壤条件下,采用肥料田间试验和测试分析方法,研究了宁夏春小麦磷素利用效率及其有关性状的基因型差异。结果表明,不论施磷与否,小麦籽粒产量、生物学产量、磷肥增产率、地上部茎叶和籽粒中含磷量以及磷素吸收累积量、磷素利用效率等均存在明显基因型差异,且这种差异在低磷条件下更为明显。在低磷条件下,籽粒生产中的磷素利用效率变化在160.1～448.3kg/kg之间,平均为223.1kg/kg,变异系数为23.05％;干物质生产中的磷素利用效率变化在332.6～898.5kg/kg之间,平均为458.4kg/kg,变异系数为22.57％。从中筛选出低磷高产高效品种8个。在较高供磷条件下,籽粒生产中的磷素利用效率变化在150.9～350.9kg/kg之间,平均为229.8kg/kg,变异系数为20.63％;于物质生产中的成素利用效率变化在358.3～746.5 kg/kg之间,平均为482.Ikg地,变异系数为 18.76％。从中筛选出高磷高产高效品种7个。通过相关分析,研究了不同供成条件下磷素利用效率与其它营养性状之间的关系,提出了培育磷高效品种的有用性状指标。     

Mast cell tryptase and asthma

As an important mediator of allergic inflammation, mast cell tryptase is involved in the in duction of hypersensitivity, infiltration of inflammatory cel s and t issue remodeling in respiratory tract.The effects of tryptase inhibitors on the actions of tryptase show further the potential of tryptase in the pathogenesis of asthma and its inhibitors in the treatment of asthma.     

Bcl-2 antisense oligodeoxynucleotide increases the sensitivity of HL60 and K562 cells to daunorubicin

AIM:To investigate whether the bcl-2 antisense oligonucleotide increases the sensitivity of HL60 and K562 cell lines to daunorubicin.METHODS:IC50 for HL60 and K562 was determined with MTT method, the expression levels of Bcl-2 protein were assayed by immunofluorescence using fluoresce isothiocyanate labeling. In addition, apoptosis was detected by morphological observation and flow cytometric analysis of DNA fragmentation.RESULTS:It was found that the two oligonucleotides directed against the coding region and the translation initiation of bcl-2 mRNA, combined respectively with daunorubicin, inhibited expression of bcl-2 protein, increased apoptosis in HL60 and K562 cells, and decreased IC50 of daunorubicin significantly (P＜0.05). Compared to the antisense oligonucleotide directed against the translation initiation of bcl-2 mRNA, the antisense oligonucleotide directed against the coding region showed stronger effects in the aspects of increasing the sensitivity of HL60 cells to daunorubicin (P＜0.05).CONCLUSIONS:These two antisense sequences in the translation initiation and the coding region of bcl-2 mRNA increased the sensitivity of HL60 and K562 cell lines to daunorubicin in a sequence-specific manner.     

Selection and amplification of the liver stem cell subset from rat bone marrow cells with a medium containing cholestatic serum in vitro

AIM: To explore the feasibility of direct separat and selective enlargement of the bone marrow-derived liver stem cells (BDLSC) from bone marrow cells with a culture system containing cholestatic serum in vitro. METHODS: Bone marrow cells of rats were cultured with selective media containing 2%, 5%, 7% and 10% cholestatic rat serum, respectively. The BDLSC were then induced to proliferate with the addition of hepatocyte growth factor (HGF) on the firth day. BDLSC were characterized using immunocytochemistry and RT-PCR for lineage markers, glycogen staining and urea synthetic assay for functions 2 weeks later. RESULTS: Bone marrow cells were unble to form colony in the presence of 2% cholestatic serum and apopotosis appeared gradually in 7% or 10% cholestatic serum. The BDLSC survived in the medium containing 5% cholestatic serum while the other types of cells did not. The survival cells proliferated with a high speed during the second week and then formed hepatocyte-like colony-forming units (H-CFU). Cells in the H-CFU expressed the characteristic proteins of fetal hepatocytes. Furthermore, they had glycogen storage and urea synthesis functions, two of the critical features of hepatocytes. CONCLUSION: The selective micro-environment effectively selected BDLSC from the bone marrow cell, and will be a new way to provide an abundant source of donor hepatocytes for clinical cell therapy.     

20%多效唑·甲哌 FEE7 微乳剂防止小麦倒伏和增产机理研究

冬小麦二棱期喷施植物生长调节剂20%多效唑·甲哌鎓微乳剂375 mL/hm2,可以显著抑制茎秆基部节间伸长,增加各节间充实度,其中赤霉素(Gas)和生长素(IAA)降低,可显著增强小麦抗倒伏能力和降低田间倒伏率.处理还协调了穗数、穗粒数和粒重的关系,增产幅度6.2%～28.6%.增产原因可能在于促进籽粒灌浆强,增加籽粒中内源Gas、IAA、细胞分裂素(CTKs)的水平,增强了籽粒库活性,同时促进茎叶中干物质向籽粒运转.